Single-cell RNA-seq

""

2024 dates(s):

Oct 14 & 15 – 9:30am – 5:00pm – in person

Notes:
workshop spans 2 full days.
– familiarity with R strongly encouraged.
New in Fall 2024

REGISTER from calendar on Workshop page.

Note: It is necessary to register for each workshop separately.

Single Cell RNA-seq Data Analysis


Description

Important note: workshop spans 2 full days.

Join the UWBC Bioinformatics Research Center for a 2-day workshop covering the fundamentals of single-cell RNA-seq data analysis.

Learn how to turn raw data into biological insight with hands-on analysis of real datasets using one of the most popular scRNA-seq analysis packages: Seurat for the R programming language. Other software options may be reviewed such as R-based Bioconductor and Python-based scanpymodule.

We will begin with tips for experimental design and an overview of single-cell technologies (e.g. Drop-seq, SMART-seq, SPLiT-Seq.)

We’ll then continue through a standard analysis workflow covering:

– quality control,
– visualization,
– integration,
– cell type identification, and
– differential expression.

At the end of this workshop, participants will have the knowledge and confidence to begin analyzing their own scRNA-seq data and pursue self-directed learning of more advanced analysis techniques.

To be prepared for this workshop, participants should have a working proficiency in R and a basic understanding of why we do single-cell experiments.